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pZ005 (AacC2c1 human codon optimized protein)
(Plasmid #70171)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70171 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    BPV00356 pET21GG2-His(6)-MBP_Flag-Avi
  • Backbone manufacturer
    Zhang Lab
  • Total vector size (bp) 10239

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AacC2c1 protein
  • Alt name
    AacCas12b
  • Species
    alicyclobacillus acidoterrestris
  • Insert Size (bp)
    3384
  • Tag / Fusion Protein
    • NLS (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZ005 (AacC2c1 human codon optimized protein) was a gift from Feng Zhang (Addgene plasmid # 70171 ; http://n2t.net/addgene:70171 ; RRID:Addgene_70171)

  • For your References section:

    Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems. Shmakov S, Abudayyeh OO, Makarova KS, Wolf YI, Gootenberg JS, Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F, Koonin EV. Mol Cell. 2015 Nov 5;60(3):385-97. doi: 10.1016/j.molcel.2015.10.008. Epub 2015 Oct 22. 10.1016/j.molcel.2015.10.008 PubMed 26593719
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