pZ004 (LseC2c2 locus)
(Plasmid
#70170)
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PurposeExpresses LseC2c2 locus with one spacer in array
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 70170 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28a
- Backbone size w/o insert (bp) 5369
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl4
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLseC2c2 locus with one spacer in array
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Specieslisteria seeligeri
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Insert Size (bp)6098
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GenBank IDWP_012985477.1
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pZ004 (LseC2c2 locus) was a gift from Feng Zhang (Addgene plasmid # 70170 ; http://n2t.net/addgene:70170 ; RRID:Addgene_70170) -
For your References section:
Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems. Shmakov S, Abudayyeh OO, Makarova KS, Wolf YI, Gootenberg JS, Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F, Koonin EV. Mol Cell. 2015 Nov 5;60(3):385-97. doi: 10.1016/j.molcel.2015.10.008. Epub 2015 Oct 22. 10.1016/j.molcel.2015.10.008 PubMed 26593719