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Addgene

pET-G2K
(Plasmid #70169)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70169 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET101/Directional-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5800
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    garK/G2K
  • Alt name
    d-glycerate 2-kinase
  • Species
    E.coli
  • Tags / Fusion Proteins
    • V5 (C terminal on backbone)
    • 6xHIS (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-G2K was a gift from George Reed & Paul Sims (Addgene plasmid # 70169 ; http://n2t.net/addgene:70169 ; RRID:Addgene_70169)

  • For your References section:

    Method for the enzymatic synthesis of 2-phospho-d-glycerate from adenosine 5′-triphosphate and d-glycerate via d-glycerate-2-kinase. Sims, P and Reed, G. Journal of Molecular Catalysis B: Enzymatic 32 (2005) 77–81 10.1016/j.molcatb.2004.10.002
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