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Addgene

pZ003 (LshC2c2 locus)
(Plasmid #70168)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70168 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pACYC184
  • Backbone manufacturer
    NEB
  • Backbone size w/o insert (bp) 4245
  • Total vector size (bp) 10657
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    LshC2c2 full locus
  • Species
    leptotrichia shahii
  • Insert Size (bp)
    6037
  • GenBank ID
    WP_018451595.1

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZ003 (LshC2c2 locus) was a gift from Feng Zhang (Addgene plasmid # 70168 ; http://n2t.net/addgene:70168 ; RRID:Addgene_70168)
  • For your References section:

    Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems. Shmakov S, Abudayyeh OO, Makarova KS, Wolf YI, Gootenberg JS, Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F, Koonin EV. Mol Cell. 2015 Nov 5;60(3):385-97. doi: 10.1016/j.molcel.2015.10.008. Epub 2015 Oct 22. 10.1016/j.molcel.2015.10.008 PubMed 26593719