YCp33-YDL183c-HA
(Plasmid
#70164)
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Purposeyeast expression of YDL183c from its own promoter and in fusion with a triple HA epitope at the C-terminal end
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 70164 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneYCp33-HA
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameYDL183c
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SpeciesS. cerevisiae (budding yeast)
- Promoter YDL183c (217 nucleotides upstream of the ATG)
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Tag
/ Fusion Protein
- 3xHA (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Sacl (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer M13F20
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
YCp33-YDL183c-HA was a gift from Karin Nowikovsky (Addgene plasmid # 70164 ; http://n2t.net/addgene:70164 ; RRID:Addgene_70164) -
For your References section:
Novel components of an active mitochondrial K(+)/H(+) exchange. Zotova L, Aleschko M, Sponder G, Baumgartner R, Reipert S, Prinz M, Schweyen RJ, Nowikovsky K. J Biol Chem. 2010 May 7;285(19):14399-414. doi: 10.1074/jbc.M109.059956. Epub 2010 Mar 2. 10.1074/jbc.M109.059956 PubMed 20197279