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pNUT N6His mICA NG
(Plasmid #70162)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70162 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNUT
  • Backbone size w/o insert (bp) 7400
  • Total vector size (bp) 9500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Methotrexate

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mouse inhibitor of carbonic anhydrase
  • Alt name
    mICA
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2136
  • Mutation
    Asn470Asp and Asn645Asp
  • GenBank ID
    NP08194
  • Promoter SV40
  • Tag / Fusion Protein
    • N-terminal signal peptide, 4 aa link, 6 His, Factor Xa cleavage site

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (not destroyed)
  • 3′ cloning site SmaI (not destroyed)
  • 5′ sequencing primer CCTTTGCGCCCGGACTCG
  • 3′ sequencing primer GTGGCAACTTCCAGGGCC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The original clone was from the laboratory of Dr. Nancy Andrews then at Harvard Medical School. It was sent to the laboratory of Dr. R.T.A. MacGillivray at the University of British Columbia. It was inserted into the pNUT vector at UBC and sent to my lab for expression, purification and characterization.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNUT N6His mICA NG was a gift from Ross MacGillivray & Anne Mason (Addgene plasmid # 70162 ; http://n2t.net/addgene:70162 ; RRID:Addgene_70162)

  • For your References section:

    A novel murine protein with no effect on iron homoeostasis is homologous with transferrin and is the putative inhibitor of carbonic anhydrase. Wang F, Lothrop AP, James NG, Griffiths TA, Lambert LA, Leverence R, Kaltashov IA, Andrews NC, MacGillivray RT, Mason AB. Biochem J. 2007 Aug 15;406(1):85-95. 10.1042/BJ20070384 PubMed 17511619
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