pEGFP-C1-MKLP1 S710A
(Plasmid #70149)

• Purpose: mammalian expression of MKLP1 with an eGFP fusion and an S710A mutation
• Depositing Lab: Masanori Mishima
• Publication: Douglas et al Curr Biol. 2010 May 25;20(10):927-33. doi: 10.1016/j.cub.2010.03.055. Epub 2010 May 6.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 7283
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MKLP1
• Alt name: kinesin family member 23
• Alt name: KIF23
• Species: H. sapiens (human)
• Insert Size (bp): 2574
• Mutation: S710A
• Entrez Gene: KIF23 (a.k.a. CDA3, CDAIII, CDAN3, CDAN3A, CHO1, KNSL5, MKLP-1, MKLP1)
• Promoter: CMV
• Tag / Fusion Protein:
  • eGFP (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • pEGFP-C1-MKLP1_S710A Genbank File

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that there are two silent mutations at position 2682 and 4212. The former is an artificial SalI site and the latter a polymorphism (I think). They also have Gly (GGG) artificially inserted between the starting methionine and the lysine 2.