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Addgene

GAPDH-CPG-TV
(Plasmid #70148)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70148 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    topo
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 3952
  • Total vector size (bp) 9370
  • Vector type
    Mouse Targeting, Synthetic Biology
  • Selectable markers
    Puromycin ; R166H in the PuroR. Puro selection has been confirmed to work well in mouse ES cells and fibroblasts.

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    5'hGapdh-Snrp-GFP-polyA-PGK-PURO-3'hGapdh
  • Species
    Synthetic

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GAPDH-CPG-TV was a gift from Rudolf Jaenisch (Addgene plasmid # 70148 ; http://n2t.net/addgene:70148 ; RRID:Addgene_70148)

  • For your References section:

    Tracing Dynamic Changes of DNA Methylation at Single-Cell Resolution. Stelzer Y, Shivalila CS, Soldner F, Markoulaki S, Jaenisch R. Cell. 2015 Sep 24;163(1):218-29. doi: 10.1016/j.cell.2015.08.046. 10.1016/j.cell.2015.08.046 PubMed 26406378
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