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Addgene

pDONR221-10X-tRNA
(Plasmid #70123)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70123 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR221
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4762
  • Total vector size (bp) 3600
  • Modifications to backbone
    Gateway BP reaction to incorporate 10X tRNA block

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    10X-tRNA block
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1100
  • Promoter None – SUP4 pol III promoter provided by insert

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The depositing lab notes that, despite the differences found in Addgene's QC sequence, this plasmid should work as advertised.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR221-10X-tRNA was a gift from Jay Hesselberth (Addgene plasmid # 70123 ; http://n2t.net/addgene:70123 ; RRID:Addgene_70123)

  • For your References section:

    Genetic bypass of essential RNA repair enzymes in budding yeast. Cherry PD, White LK, York K, Hesselberth JR. RNA. 2018 Mar;24(3):313-323. doi: 10.1261/rna.061788.117. Epub 2017 Dec 6. 10.1261/rna.061788.117 PubMed 29212664
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