pEMS2159
(Plasmid #70120)

• Purpose: Promoterless AAV plasmid with MCS to insert promoter for expression of iCre. Contains WPRE.
• Depositing Lab: Elizabeth Simpson
• Publication: Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118.

Backbone

• Vector backbone: AAV2
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ssAAV-MCS-iCre-WPRE
• Alt name: iCre
• Species: Synthetic
• Promoter: Promoterless

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: see map (unknown if destroyed)
• 3′ cloning site: see map (unknown if destroyed)
• 5′ sequencing primer: M13pUC-rev
• 3′ sequencing primer: EBV-rev

Resource Information

• Supplemental Documents:
  • pEMS2159 GenBank file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid