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Addgene

pAG4
(Plasmid #70115)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70115 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC18
  • Modifications to backbone
    Modified from pUC18-mini-Tn7T-Gm-lux (AY962893.1) with deletions of nt 1053-1113 and 2042-2503.
  • Vector type
    Bacterial Expression
  • Promoter none
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    H. Schweizer

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See J. Microbio. Methods 2015 118:75-79 for details
The authors used this vector along with a Tn7 transposase encoding plasmid, pTNS1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAG4 was a gift from Thomas Lewis (Addgene plasmid # 70115 ; http://n2t.net/addgene:70115 ; RRID:Addgene_70115)

  • For your References section:

    An improved Tn7-lux reporter for broad host range, chromosomally-integrated promoter fusions in Gram-negative bacteria. Glassing A, Lewis TA. J Microbiol Methods. 2015 Sep 1;118:75-77. doi: 10.1016/j.mimet.2015.08.016. 10.1016/j.mimet.2015.08.016 PubMed 26341612
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