pECFP-C1-hSERT
(Plasmid #70104)

• Purpose: mammalian expression of hSERT with N-terminal CFP fusion
• Depositing Lab: Harald Sitte
• Publication: Sucic et al J Biol Chem. 2010 Apr 2;285(14):10924-38. doi: 10.1074/jbc.M109.083154. Epub 2010 Jan 29.

Backbone

• Vector backbone: pECcFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 6600
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SERT
• Species: H. sapiens (human)
• Entrez Gene: SLC6A4 (a.k.a. 5-HTT, 5-HTTLPR, 5HTT, HTT, OCD1, SERT, SERT1, hSERT)
• Promoter: CMV
• Tag / Fusion Protein:
  • ECFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry