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DGP104- pSP64 Poly(A)700
(Plasmid #70098)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70098 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSP64
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2999
  • Total vector size (bp) 3700
  • Vector type
    in vitro transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    700bp insert from B. subtilis
  • Species
    B. subtilis
  • Promoter SP6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer M13-Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DGP104- pSP64 Poly(A)700 was a gift from David Gresham (Addgene plasmid # 70098 ; http://n2t.net/addgene:70098 ; RRID:Addgene_70098)

  • For your References section:

    Determination of in vivo RNA kinetics using RATE-seq. Neymotin B, Athanasiadou R, Gresham D. RNA. 2014 Oct;20(10):1645-52. doi: 10.1261/rna.045104.114. Epub 2014 Aug 26. 10.1261/rna.045104.114 PubMed 25161313
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