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pLKO_FOXA1_#2
(Plasmid #70096)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70096 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLKO.1
  • Backbone manufacturer
    Broad Institute
  • Backbone size w/o insert (bp) 7000
  • Total vector size (bp) 7000
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FOXA1
  • gRNA/shRNA sequence
    GCGTACTACCAAGGTGTGTAT
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_004496.3
  • Entrez Gene
    FOXA1 (a.k.a. HNF3A, TCF3A)
  • Promoter u6

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Broad Institute
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Broad Institute Clone ID TRCN0000014879

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO_FOXA1_#2 was a gift from William Hahn (Addgene plasmid # 70096 ; http://n2t.net/addgene:70096 ; RRID:Addgene_70096)

  • For your References section:

    The androgen receptor cistrome is extensively reprogrammed in human prostate tumorigenesis. Pomerantz MM, Li F, Takeda DY, Lenci R, Chonkar A, Chabot M, Cejas P, Vazquez F, Cook J, Shivdasani RA, Bowden M, Lis R, Hahn WC, Kantoff PW, Brown M, Loda M, Long HW, Freedman ML. Nat Genet. 2015 Nov;47(11):1346-51. doi: 10.1038/ng.3419. Epub 2015 Oct 12. 10.1038/ng.3419 PubMed 26457646
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