pLX302_FOXA1-V5
(Plasmid #70090)

• Purpose: Expression of human FOXA1 with C-terminal V5 tag, puromycin selection marker, 3rd generation lentiviral vector
• Depositing Lab: William Hahn
• Publication: Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi: 10.1038/ng.3419. Epub 2015 Oct 12.

Backbone

• Vector backbone: pLX_TRC302
• Backbone size w/o insert (bp): 9573
• Total vector size (bp): 9334
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: FOXA1
• Alt name: HNF3A
• Alt name: TCF3A
• Species: H. sapiens (human)
• Insert Size (bp): 1400
• GenBank ID: NM_004496.3
• Entrez Gene: FOXA1 (a.k.a. HNF3A, TCF3A)
• Promoter: CMV
• Tag / Fusion Protein:
  • V5 (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: pLX_forward
• 3′ sequencing primer: pLX_reverse

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please Note- Some scientists have observed an incorrect digest pattern using NotI. Addgene has verified by sequencing that a NotI site is present within the FoxA1 insert.