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PurposeExpression of human HOXB13 from CMV promoter, puromycin selection marker
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 70089 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLX_TRC302
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Backbone manufacturerBroad Institute
- Backbone size w/o insert (bp) 9573
- Total vector size (bp) 8776
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHOXB13
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SpeciesH. sapiens (human)
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Insert Size (bp)900
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GenBank IDNM_006361.5
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Entrez GeneHOXB13 (a.k.a. HPC9, PSGD)
- Promoter CMV
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer pLX_forward
- 3′ sequencing primer pLX_reverse (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLX302_HOXB13 was a gift from William Hahn (Addgene plasmid # 70089 ; http://n2t.net/addgene:70089 ; RRID:Addgene_70089) -
For your References section:
The androgen receptor cistrome is extensively reprogrammed in human prostate tumorigenesis. Pomerantz MM, Li F, Takeda DY, Lenci R, Chonkar A, Chabot M, Cejas P, Vazquez F, Cook J, Shivdasani RA, Bowden M, Lis R, Hahn WC, Kantoff PW, Brown M, Loda M, Long HW, Freedman ML. Nat Genet. 2015 Nov;47(11):1346-51. doi: 10.1038/ng.3419. Epub 2015 Oct 12. 10.1038/ng.3419 PubMed 26457646