pNUT N6His Y95F/Y188F/Y426F/Y517F hTFNG hTFNG
(Plasmid #70085)

• Purpose: Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in either the N-lobe or the C-lobeN-lobe
• Depositing Labs: Ross MacGillivray, Anne Mason
• Publication: Mason et al Protein Expr Purif. 2004 Aug;36(2):318-26.

Backbone

• Vector backbone: pNUT
• Backbone size w/o insert (bp): 7400
• Total vector size (bp): 9700
• Vector type: Mammalian Expression
• Selectable markers: Methotrexate

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mutated human serum transferrin
• Alt name: Y95F/Y188F/Y426F/Y517F hTF NG
• Alt name: Authentic apo TF
• Species: H. sapiens (human)
• Insert Size (bp): 2136
• Mutation: Asn413 Asp, Asn611Asp, Tyr95Phe, Tyr188Phe, Ty426Phe, Tyr517Phe
• GenBank ID: AF28814 PRO1557, PRO2086, TFQTL1
• Entrez Gene: TF (a.k.a. HEL-S-71p, PRO1557, PRO2086, TFQTL1)
• Promoter: SV40
• Tags / Fusion Proteins:
  • N-terminal signal peptide, 4 aa link, 6 His, Factor Xa cleavage site (N terminal on insert)
  • Hexa His tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SmaI (not destroyed)
• 3′ cloning site: SmaI (not destroyed)
• 5′ sequencing primer: CCTTTGCGCCCGGACTCG
• 3′ sequencing primer: GTGGCAACTTCCAGGGCC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr Ross T.A. MacGillivray UBC Vancouver BC Canada

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry