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pNUT N6His Y95F/Y188F/Y426F/Y517F hTFNG hTFNG
(Plasmid #70085)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70085 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNUT
  • Backbone size w/o insert (bp) 7400
  • Total vector size (bp) 9700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Methotrexate

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mutated human serum transferrin
  • Alt name
    Y95F/Y188F/Y426F/Y517F hTF NG
  • Alt name
    Authentic apo TF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2136
  • Mutation
    Asn413 Asp, Asn611Asp, Tyr95Phe, Tyr188Phe, Ty426Phe, Tyr517Phe
  • GenBank ID
    AF28814 PRO1557, PRO2086, TFQTL1
  • Entrez Gene
    TF (a.k.a. HEL-S-71p, PRO1557, PRO2086, TFQTL1)
  • Promoter SV40
  • Tags / Fusion Proteins
    • N-terminal signal peptide, 4 aa link, 6 His, Factor Xa cleavage site (N terminal on insert)
    • Hexa His tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (not destroyed)
  • 3′ cloning site SmaI (not destroyed)
  • 5′ sequencing primer CCTTTGCGCCCGGACTCG
  • 3′ sequencing primer GTGGCAACTTCCAGGGCC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr Ross T.A. MacGillivray UBC Vancouver BC Canada

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNUT N6His Y95F/Y188F/Y426F/Y517F hTFNG hTFNG was a gift from Ross MacGillivray & Anne Mason (Addgene plasmid # 70085 ; http://n2t.net/addgene:70085 ; RRID:Addgene_70085)

  • For your References section:

    Expression, purification, and characterization of authentic monoferric and apo-human serum transferrins. Mason AB, Halbrooks PJ, Larouche JR, Briggs SK, Moffett ML, Ramsey JE, Connolly SA, Smith VC, MacGillivray RT. Protein Expr Purif. 2004 Aug;36(2):318-26. 10.1016/j.pep.2004.04.013 PubMed 15249056
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