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pET11d-FHis-DND1-Y102A
(Plasmid #70082)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70082 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-11d
  • Backbone manufacturer
    EMD Millipore
  • Backbone size w/o insert (bp) 5640
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Dnd1
  • Alt name
    dead end homolog 1
  • Alt name
    RBMS4
  • Alt name
    DND microRNA-mediated repression inhibitor 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1134
  • Mutation
    Y102A. Codon optimized for bacterial expression
  • GenBank ID
    213236
  • Entrez Gene
    DND1 (a.k.a. RBMS4)
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • 6xHIS (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminal
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET11d-FHis-DND1-Y102A was a gift from Thomas Tuschl (Addgene plasmid # 70082 ; http://n2t.net/addgene:70082 ; RRID:Addgene_70082)

  • For your References section:

    DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs. Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, Garzia A, Morozov P, Manickavel S, McFarland HL, Roeder RG, Hafner M, Tuschl T. Nature. 2017 Mar 23;543(7646):568-572. doi: 10.1038/nature21690. Epub 2017 Mar 15. 10.1038/nature21690 PubMed 28297718
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