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Addgene

pLVE-FH-Dnd1-IN
(Plasmid #70072)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70072 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVE-FH-IN
  • Backbone manufacturer
    Tuschl lab, Addgene plasmid #70071
  • Backbone size w/o insert (bp) 10748
  • Vector type
    Mammalian Expression, Lentiviral, Cre/Lox
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Dnd1
  • Alt name
    dead end homolog 1
  • Alt name
    RBMS4
  • Alt name
    DND microRNA-mediated repression inhibitor 1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1065
  • GenBank ID
    213236
  • Entrez Gene
    Dnd1 (a.k.a. RBMS4, Ter)
  • Promoter EF1alpha
  • Tags / Fusion Proteins
    • FLAG (N terminal on backbone)
    • HA (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVE-FH-Dnd1-IN was a gift from Thomas Tuschl (Addgene plasmid # 70072 ; http://n2t.net/addgene:70072 ; RRID:Addgene_70072)

  • For your References section:

    DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs. Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, Garzia A, Morozov P, Manickavel S, McFarland HL, Roeder RG, Hafner M, Tuschl T. Nature. 2017 Mar 23;543(7646):568-572. doi: 10.1038/nature21690. Epub 2017 Mar 15. 10.1038/nature21690 PubMed 28297718
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