pENTR4-hDND1
(Plasmid
#70070)
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PurposeGateway entry vector for human DND1 with stop codon
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 70070 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR221
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Backbone manufacturerLife Technologies
- Backbone size w/o insert (bp) 2567
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Vector typegateway entry vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameDnd1
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Alt namedead end homolog 1
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Alt nameRBMS4
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Alt nameDND microRNA-mediated repression inhibitor 1
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SpeciesH. sapiens (human)
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Insert Size (bp)1088
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Entrez GeneDND1 (a.k.a. RBMS4)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F20
- 3′ sequencing primer M13R, T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR4-hDND1 was a gift from Thomas Tuschl (Addgene plasmid # 70070 ; http://n2t.net/addgene:70070 ; RRID:Addgene_70070) -
For your References section:
DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs. Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, Garzia A, Morozov P, Manickavel S, McFarland HL, Roeder RG, Hafner M, Tuschl T. Nature. 2017 Mar 23;543(7646):568-572. doi: 10.1038/nature21690. Epub 2017 Mar 15. 10.1038/nature21690 PubMed 28297718