pTH07
(Plasmid
#70063)
-
Purposeartificial transcription template "distal pause"
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 70063 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepFW11 Tet
Growth in Bacteria
-
Bacterial Resistance(s)Chloramphenicol and Tetracycline, 25 & 10 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namelambda PR' - repeat cassette - E. coli rpoB with inserted pause - lambda TR'
-
SpeciesSynthetic
-
GenBank IDKT326915
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pTH07 was a gift from Jeff Gelles (Addgene plasmid # 70063 ; http://n2t.net/addgene:70063 ; RRID:Addgene_70063) -
For your References section:
Bacterial RNA polymerase can retain sigma70 throughout transcription. Harden TT, Wells CD, Friedman LJ, Landick R, Hochschild A, Kondev J, Gelles J. Proc Natl Acad Sci U S A. 2016 Jan 5. pii: 201513899. 10.1073/pnas.1513899113 PubMed 26733675
Map uploaded by the depositor.