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Addgene

pCDW114
(Plasmid #70061)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70061 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFW11 Tet

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Tetracycline, 25 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    lambda PR' - repeat cassette - E. coli rpoB - lambda TR'
  • Species
    Synthetic
  • GenBank ID
    KT326913

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDW114 was a gift from Jeff Gelles (Addgene plasmid # 70061 ; http://n2t.net/addgene:70061 ; RRID:Addgene_70061)

  • For your References section:

    Bacterial RNA polymerase can retain sigma70 throughout transcription. Harden TT, Wells CD, Friedman LJ, Landick R, Hochschild A, Kondev J, Gelles J. Proc Natl Acad Sci U S A. 2016 Jan 5. pii: 201513899. 10.1073/pnas.1513899113 PubMed 26733675
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