AP582-2
(Plasmid
#70049)
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PurposeExpresses Ollas::mCherry::linker::H2B::V5 in bacteria
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 70049 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC19
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Backbone manufacturerMessing lab Plasmid 50005
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemCherry::linker::H2B
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SpeciesC. elegans (nematode)
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Tags
/ Fusion Proteins
- Ollas (N terminal on insert)
- V5 (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13pUC-Fwd
- 3′ sequencing primer M13pUC-Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AP582-2 was a gift from Geraldine Seydoux (Addgene plasmid # 70049 ; http://n2t.net/addgene:70049 ; RRID:Addgene_70049) -
For your References section:
High Efficiency, Homology-Directed Genome Editing in Caenorhabditis elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes. Paix A, Folkmann A, Rasoloson D, Seydoux G. Genetics. 2015 Sep;201(1):47-54. doi: 10.1534/genetics.115.179382. Epub 2015 Jul 17. 10.1534/genetics.115.179382 PubMed 26187122
