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MIG-eIF4A1 Q195E
(Plasmid #70044)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70044 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV IRES GFP (MIG)
  • Backbone size w/o insert (bp) 6500
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eIF4A1
  • Alt name
    DDX2A
  • Alt name
    EIF4A
  • Alt name
    EIF-4A
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1400
  • Mutation
    Q195E
  • GenBank ID
    NM_144958.4 1973
  • Entrez Gene
    Eif4a1 (a.k.a. BM-010, Ddx2a, Eif4)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pLXSN 5'
  • 3′ sequencing primer pCDH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MIG-eIF4A1 Q195E was a gift from Hans-Guido Wendel (Addgene plasmid # 70044 ; http://n2t.net/addgene:70044 ; RRID:Addgene_70044)

  • For your References section:

    RNA G-quadruplexes cause eIF4A-dependent oncogene translation in cancer. Wolfe AL, Singh K, Zhong Y, Drewe P, Rajasekhar VK, Sanghvi VR, Mavrakis KJ, Jiang M, Roderick JE, Van der Meulen J, Schatz JH, Rodrigo CM, Zhao C, Rondou P, de Stanchina E, Teruya-Feldstein J, Kelliher MA, Speleman F, Porco JA Jr, Pelletier J, Ratsch G, Wendel HG. Nature. 2014 Sep 4;513(7516):65-70. doi: 10.1038/nature13485. Epub 2014 Jul 27. 10.1038/nature13485 PubMed 25079319
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