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Addgene

PGL4-4X TE Down (GQs) Renilla
(Plasmid #70039)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70039 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL4.13
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4600
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    4x TE Down (G-quadruplexes) in 5'UTR
  • Alt name
    Four tandem repeats of the (CGG)4 12-mer motif
  • Species
    Synthetic
  • Insert Size (bp)
    100
  • Mutation
    4 repeats of TE down motif (G-quadruplexes) in 5'UTR
  • Promoter SV40
  • Tag / Fusion Protein
    • renilla luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AvrII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer SV40pro-F
  • 3′ sequencing primer Luc2-Rev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PGL4-4X TE Down (GQs) Renilla was a gift from Hans-Guido Wendel (Addgene plasmid # 70039 ; http://n2t.net/addgene:70039 ; RRID:Addgene_70039)

  • For your References section:

    RNA G-quadruplexes cause eIF4A-dependent oncogene translation in cancer. Wolfe AL, Singh K, Zhong Y, Drewe P, Rajasekhar VK, Sanghvi VR, Mavrakis KJ, Jiang M, Roderick JE, Van der Meulen J, Schatz JH, Rodrigo CM, Zhao C, Rondou P, de Stanchina E, Teruya-Feldstein J, Kelliher MA, Speleman F, Porco JA Jr, Pelletier J, Ratsch G, Wendel HG. Nature. 2014 Sep 4;513(7516):65-70. doi: 10.1038/nature13485. Epub 2014 Jul 27. 10.1038/nature13485 PubMed 25079319
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