pUC19-TSV (linearized in LT)
(Plasmid #70033)

• Purpose: pUC19-TSV (linearized in LT) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the Large T-antigen coding region
• Depositing Lab: Mariet Feltkamp
• Publication: van der Meijden et al PLoS Pathog. 2010 Jul 29;6(7):e1001024. doi: 10.1371/journal.ppat.1001024.

Backbone

• Vector backbone: pUC19
• Backbone size w/o insert (bp): 2686
• Total vector size (bp): 7918
• Vector type: Bacterial Expression ; Cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TSPyV full genome, linearized in Large T-antigen
• Alt name: Trichodysplasia Spinulosa-associated polyomavirus
• Alt name: Common abbreviation: TSV; TSPyV
• Species: Polyomavirus
• Insert Size (bp): 5232
• GenBank ID: NC_014361

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry