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Addgene

pUC19-TSV (linearized in VP2)
(Plasmid #70032)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70032 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2686
  • Total vector size (bp) 7918
  • Vector type
    Bacterial Expression ; Cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TSV full genome, linearized in VP2 gene
  • Alt name
    Trichodysplasia spinulosa-associated polyomavirus
  • Alt name
    Common abbreviations: TSV; TSPyV
  • Species
    Trichodysplasia Spinulosa-associated Polyomavirus
  • Insert Size (bp)
    5232
  • GenBank ID
    NC_014361

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 3′ cloning site SmaI (destroyed during cloning)
  • 5′ sequencing primer -
  • 3′ sequencing primer -
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC19-TSV (linearized in VP2) was a gift from Mariet Feltkamp (Addgene plasmid # 70032 ; http://n2t.net/addgene:70032 ; RRID:Addgene_70032)

  • For your References section:

    Discovery of a new human polyomavirus associated with trichodysplasia spinulosa in an immunocompromized patient. van der Meijden E, Janssens RW, Lauber C, Bouwes Bavinck JN, Gorbalenya AE, Feltkamp MC. PLoS Pathog. 2010 Jul 29;6(7):e1001024. doi: 10.1371/journal.ppat.1001024. 10.1371/journal.ppat.1001024 PubMed 20686659
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