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PurposeCas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 7
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 70019 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLSLR
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Backbone manufacturern/a
- Total vector size (bp) 17961
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Vector typePlant Expression, CRISPR
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Selectable markerskanamycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNuclease (Cas9/sgRNA) + Donor + GVR
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SpeciesSolanum lycopersicum
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Insert Size (bp)11728
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTC223 was a gift from Daniel Voytas (Addgene plasmid # 70019 ; http://n2t.net/addgene:70019 ; RRID:Addgene_70019) -
For your References section:
High-frequency, precise modification of the tomato genome. Cermak T, Baltes NJ, Cegan R, Zhang Y, Voytas DF. Genome Biol. 2015 Nov 6;16(1):232. doi: 10.1186/s13059-015-0796-9. 10.1186/s13059-015-0796-9 [pii] PubMed 26541286