pTC147
(Plasmid
#70013)
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PurposeT-DNA vector with Kanamycin resistance carrying a 35S:ANT1 over-expression cassette.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 70013 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCambia1302
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Backbone manufacturern/a
- Total vector size (bp) 10606
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Vector typePlant Expression
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Selectable markerskanamycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name35S:ANT1
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SpeciesSolanum lycopersicum
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Insert Size (bp)1855
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTC147 was a gift from Daniel Voytas (Addgene plasmid # 70013 ; http://n2t.net/addgene:70013 ; RRID:Addgene_70013) -
For your References section:
High-frequency, precise modification of the tomato genome. Cermak T, Baltes NJ, Cegan R, Zhang Y, Voytas DF. Genome Biol. 2015 Nov 6;16(1):232. doi: 10.1186/s13059-015-0796-9. 10.1186/s13059-015-0796-9 [pii] PubMed 26541286