pER-eZinCh-2
(Plasmid
#70009)
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Purposeexpresses ER-eZinCh-2 Zn2+ FRET sensor in the ER of mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 70009 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsE. coli nova blue LB culture medium with ampicillin. Culture overnight, 37 C/250 rpm
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameER-eZinCh-2
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SpeciesSynthetic
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Insert Size (bp)1700
- Promoter CMV
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Tags
/ Fusion Proteins
- PPI signal (N terminal on insert)
- KDEL sequence (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV forward
- 3′ sequencing primer CMV reversed (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pER-eZinCh-2 was a gift from Maarten Merkx (Addgene plasmid # 70009 ; http://n2t.net/addgene:70009 ; RRID:Addgene_70009) -
For your References section:
eZinCh-2: A Versatile, Genetically Encoded FRET Sensor for Cytosolic and Intraorganelle Zn(2+) Imaging. Hessels AM, Chabosseau P, Bakker MH, Engelen W, Rutter GA, Taylor KM, Merkx M. ACS Chem Biol. 2015 Sep 18;10(9):2126-34. doi: 10.1021/acschembio.5b00211. Epub 2015 Jul 20. 10.1021/acschembio.5b00211 PubMed 26151333
