peZinCh-2
(Plasmid #70008)

• Purpose: expresses eZinCh-2 Zn2+ FRET sensor in the cytosol of mammalian cells
• Depositing Lab: Maarten Merkx
• Publication: Hessels et al ACS Chem Biol. 2015 Sep 18;10(9):2126-34. doi: 10.1021/acschembio.5b00211. Epub 2015 Jul 20.

Backbone

• Vector backbone: pCMV
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3900
• Total vector size (bp): 5600
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: E. coli nova blue LB culture medium 30 ug/ml kanamycin Culture overnight, 37 C/250 rpm
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: eZinCh-2
• Insert Size (bp): 1600
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV promotor
• 3′ sequencing primer: SV40pA-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry