pGM202T7
(Plasmid #69993)

• Purpose: E. coli/Streptomyces expression vector containing the T7-promoter
• Depositing Lab: Günther Muth
• Publication: Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(21):9067-9080. doi: 10.1007/s00253-018-9334-5. Epub 2018 Sep 6.

Backbone

• Vector backbone: pSG5
• Backbone size w/o insert (bp): 2140
• Total vector size (bp): 6537
• Vector type: Bacterial Expression
• Selectable markers: kanamycin, thiostrepton

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Replicates also in Streptomyces strains ( below 35°C), selection kanamycin or thiostrepton
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: aphII
• Alt name: kan, neo
• Species: Transposon Tn5
• Insert Size (bp): 794

Gene/Insert 2

• Gene/Insert name: tsr
• Alt name: thiostrepton resistance
• Species: Streptomyces azureus
• Insert Size (bp): 809
• GenBank ID: X02392

Gene/Insert 3

• Gene/Insert name: rep
• Species: Streptomyces ghanaensis
• Insert Size (bp): 1442

Gene/Insert 4

• Gene/Insert name: sso
• Alt name: single stranded origin
• Species: Streptomycves ghanaensis
• Insert Size (bp): 300

Gene/Insert 5

• Gene/Insert name: T7 promoter
• Alt name: P_T7
• Species: T7 phage
• Insert Size (bp): 20

Gene/Insert 6

• Gene/Insert name: dso
• Alt name: double-stranded origin

Gene/Insert 7

• Gene/Insert name: His-Orf
• Species: Synthetic
• Insert Size (bp): 150
• Tag / Fusion Protein:
  • N-terminal and C-terminal His tag encoding sequences (N terminal on insert)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Stable replicating, multi copy shuttle vector for inducible gene expression (allowing translational fusion to N-terminal or C-terminal His-tag) in Streptomyces and E. coli. The T7 promoter requires specific host strains (eg. BL21 or S. lividans T7), providing the T7-polymerase. The vector has temperature sensitive replication (G. Muth, B. Nußbaumer, W. Wohlleben and A. Pühler (1989) A vector system with temperature-sensitive replication for gene disruption and mutational cloning in streptomycetes. Mol. Gen. Genet. 219, 341-348 )
http://www.uni-tuebingen.de/en/faculties/faculty-of-science/departments/inter-faculty-institutes-and-centres/imit/units/mikrobiologiebiotechnologie/work-groups/muth/plasmids.html