pY016 (pcDNA3.1-hLbCpf1)
(Plasmid #69988)

• Purpose: Expresses humanized LbCpf1
• Depositing Lab: Feng Zhang
• Publication: Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5354
• Total vector size (bp): 9227
• Vector type: Mammalian Expression, CRISPR
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: hLbCpf1
• Alt name: Cpf1
• Alt name: LbCas12a
• Species: Lachnospiraceae_bacterium_ND2006
• Insert Size (bp): 3684
• Promoter: CMV
• Tag / Fusion Protein:
  • NLS-3xHA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: GenScript USA Inc.
• Articles Citing this Plasmid:
  • 40 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry