pAAV-FLEX-NBL10
(Plasmid #69971)

• Purpose: Cre-conditionally expresses the anti-GFP Nanobody-Rpl10a fusion
• Depositing Lab: Jeffrey Friedman
• Publication: Ekstrand et al Cell. 2014 May 22;157(5):1230-42. doi: 10.1016/j.cell.2014.03.059.

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Backbone

• Vector backbone: pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA
• Backbone manufacturer: Karl Deisseroth
• Backbone size w/o insert (bp): 5600
• Total vector size (bp): 6672
• Modifications to backbone: n/a
• Vector type: Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: NBL10
• Alt name: Nanobody-L10
• Alt name: Anti-GFP Nanobody-Rpl10a
• Species: M. musculus (mouse); Lama pacos (camelid)
• Insert Size (bp): 1038
• Promoter: EF1a
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: oME328
• 3′ sequencing primer: oME327

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Nanobody from Rothbauer et al., 2006; L10 from Heiman et al., 2008

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See also Nectow et al., Nature Protocols (2015).