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pAAV-FLEX-NBL10
(Plasmid #69971)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69971 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA
  • Backbone manufacturer
    Karl Deisseroth
  • Backbone size w/o insert (bp) 5600
  • Total vector size (bp) 6672
  • Modifications to backbone
    n/a
  • Vector type
    Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NBL10
  • Alt name
    Nanobody-L10
  • Alt name
    Anti-GFP Nanobody-Rpl10a
  • Species
    M. musculus (mouse); Lama pacos (camelid)
  • Insert Size (bp)
    1038
  • Promoter EF1a
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer oME328
  • 3′ sequencing primer oME327
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Nanobody from Rothbauer et al., 2006; L10 from Heiman et al., 2008

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See also Nectow et al., Nature Protocols (2015).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-FLEX-NBL10 was a gift from Jeffrey Friedman (Addgene plasmid # 69971 ; http://n2t.net/addgene:69971 ; RRID:Addgene_69971)

  • For your References section:

    Molecular profiling of neurons based on connectivity. Ekstrand MI, Nectow AR, Knight ZA, Latcha KN, Pomeranz LE, Friedman JM. Cell. 2014 May 22;157(5):1230-42. doi: 10.1016/j.cell.2014.03.059. 10.1016/j.cell.2014.03.059 PubMed 24855954
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