5C7M (ITCH)
(Plasmid
#69966)
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PurposeBacterial expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 69966 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepET28-MHL
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Backbone manufacturerSGC (Addgene plasmid #26096)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameITCH:SDC094-D06:C29524
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Alt nameitch.483.862
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Alt namegi|27477108
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SpeciesH. sapiens (human)
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Entrez GeneITCH (a.k.a. ADMFD, AIF4, AIP4, NAPP1)
- Promoter T7-lacO
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Tags
/ Fusion Proteins
- His (N terminal on backbone)
- TEV cleavage site (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
http://www.thesgc.org/structures/5C7M (complex) for more information
Note the 3D structure associated with PDB ID: 5C7M was a complex generated using 5C7M (ITCH): plasmid# 69966 and 5C7M (UBC): plasmid# 69967.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
5C7M (ITCH) was a gift from Cheryl Arrowsmith (Addgene plasmid # 69966 ; http://n2t.net/addgene:69966 ; RRID:Addgene_69966)