5C7J
(Plasmid
#69965)
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PurposeBacterial expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 69965 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28-MHL
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Backbone manufacturerSGC (Addgene plasmid #26096)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNEDD4:SDC094-F02:C29544
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Alt namenedd4.0520.0900
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Alt namegi|114520608
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SpeciesH. sapiens (human)
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Entrez GeneNEDD4 (a.k.a. NEDD4-1, RPF1)
- Promoter T7-lacO
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Tags
/ Fusion Proteins
- His (N terminal on backbone)
- TEV cleavage site (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
http://www.thesgc.org/structures/5C7J for more information
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
5C7J was a gift from Cheryl Arrowsmith (Addgene plasmid # 69965 ; http://n2t.net/addgene:69965 ; RRID:Addgene_69965)