5C5C
(Plasmid
#69964)
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PurposeBacterial expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 69964 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFHMSP-LIC-N
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Backbone manufacturerSGC (Addgene plasmid #26095)
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Vector typeBaculovirus expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGRM7:DCC014-D04:C211307
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Alt nameGRM7A-s001-C249S:M37-K513
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Alt namegi|32528267
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SpeciesH. sapiens (human)
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Entrez GeneGRM7 (a.k.a. GLUR7, GPRC1G, MGLU7, MGLUR7, PPP1R87)
- Promoter polyhedrin
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Tags
/ Fusion Proteins
- His (N terminal on backbone)
- TEV cleavage site (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pFHMSP-fwd (5'-CCGGATTATTCATACCGTCCCACCA-3')
- 3′ sequencing primer pFHMSP-rev (5'-CTGATTATGATCCTCTAGTACTTCT-3') (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
http://www.thesgc.org/structures/5C5C for more information
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
5C5C was a gift from Cheryl Arrowsmith (Addgene plasmid # 69964 ; http://n2t.net/addgene:69964 ; RRID:Addgene_69964)