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Addgene

4YVD
(Plasmid #69960)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69960 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFBOH-MHL
  • Backbone manufacturer
    SGC (Addgene plasmid #62304)
  • Vector type
    Baculovirus expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PLRG1:JMC102-C09:C233452
  • Alt name
    PLRG1_BV4
  • Alt name
    gi|77404429
  • Species
    H. sapiens (human)
  • Entrez Gene
    PLRG1 (a.k.a. Cwc1, PRL1, PRP46, PRPF46, TANGO4)
  • Promoter polyhedrin
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • TEV cleavage site (N terminal on backbone)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer pFBOH-fwd (5'-CCGGATTATTCATACCGTCCCACCA-3')
  • 3′ sequencing primer pFBOH-rev (5'- CTGATTATGATCCTCTAGTACTTCT-3')
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    4YVD was a gift from Cheryl Arrowsmith (Addgene plasmid # 69960 ; http://n2t.net/addgene:69960 ; RRID:Addgene_69960)
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