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Addgene

pSNAP-CAPZ
(Plasmid #69948)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69948 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-3d
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    CapZ beta 1
  • Alt name
    capping protein
  • Species
    G. gallus (chicken)
  • Entrez Gene
    CAPZB
  • Tags / Fusion Proteins
    • His 6
    • SNAP

Gene/Insert 2

  • Gene/Insert name
    CapZ alpha 1
  • Species
    G. gallus (chicken)
  • Entrez Gene
    CAPZA1

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The SNAP tag in this plasmid has a (inadvertent) mutation L69Q. This does not seem to affect the labeling or function of the tag.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSNAP-CAPZ was a gift from Jeff Gelles (Addgene plasmid # 69948 ; http://n2t.net/addgene:69948 ; RRID:Addgene_69948)

  • For your References section:

    Single-molecule visualization of a formin-capping protein 'decision complex' at the actin filament barbed end. Bombardier JP, Eskin JA, Jaiswal R, Correa IR Jr, Xu MQ, Goode BL, Gelles J. Nat Commun. 2015 Nov 13;6:8707. doi: 10.1038/ncomms9707. 10.1038/ncomms9707 PubMed 26566078
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