taRAJ31min
(Plasmid #69934)

• Purpose: Expresses taRAJ31min, a linear riboregulator.
• Depositing Lab: Alfonso Jaramillo
• Publication: Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. doi: 10.34133/2020/1916789. eCollection 2020.

Backbone

• Vector backbone: pIDTsmartAmp
• Backbone manufacturer: IDT DNA
• Backbone size w/o insert (bp): 2118
• Total vector size (bp): 2271
• Modifications to backbone: BsaI site removed
• Vector type: Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: plTet-taRAJ31min
• Species: Synthetic
• Insert Size (bp): 153
• Promoter: PltetO1-2

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: TGTAAAACGACGGCCAGT
• 3′ sequencing primer: GTCATAGCTGTTTCCTG

Resource Information

• Supplemental Documents:
  • taRAJ31min - Genbank annotation

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Engineering a circular riboregulator in Escherichia coli - Rostain et al.
Please check Genbank file for complete annotation.