circRAJ31-Toehold_mutant
(Plasmid #69930)

• Purpose: Expresses a toehold mutant of the circRAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant does not activate cisRAJ31.
• Depositing Lab: Alfonso Jaramillo
• Publication: Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. doi: 10.34133/2020/1916789. eCollection 2020.

Backbone

• Vector backbone: pIDTsmartAmp
• Backbone manufacturer: IDT DNA
• Backbone size w/o insert (bp): 2584
• Total vector size (bp): 2106
• Modifications to backbone: BsaI site removed
• Vector type: Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: plTet-circRAJ31_toeholdmutant
• Species: Synthetic
• Insert Size (bp): 478
• Mutation: 4 bases mutated in toehold of riboregulator
• Promoter: PltetO1-2

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: TGTAAAACGACGGCCAGT
• 3′ sequencing primer: GTCATAGCTGTTTCCTG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Engineering a circular riboregulator in Escherichia coli - Rostain et al.
Please check Genbank file for complete sequence annotation.