pET28-MBP-TEV
(Plasmid #69929)

• Purpose: (Empty Backbone) bacterial expression of MBP-TEV n-terminal fusions
• Depositing Labs: Zita Balklava, Thomas Wassmer
• Publication: Currinn et al Cell Mol Life Sci. 2015 Jul 28.

Backbone

• Vector backbone: pET28
• Backbone manufacturer: Novagen
• Modifications to backbone: We cloned the ORF fragment encoding K27 to K396 of the E. coli malE protein together with a TEV protease cleavage site (ENLYFQGS) using the NheI and BamHI restriction sites of the pET28 bacterial expression plasmid (Novagen)
• Vector type: Bacterial Expression
• Promoter: T7
• Tag / Fusion Protein:
  • MBP-TEV (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminal

Resource Information

• Articles Citing this Plasmid:
  • 17 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Information for Cloning Grade DNA (Catalog # 69929-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here