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Addgene

pET28-MBP-TEV-AICD
(Plasmid #69927)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69927 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28-MBP-TEV
  • Backbone manufacturer
    Dr. Zita Balklava and Dr. Thomas Wassmer, Addgene plasmid #69929
  • Total vector size (bp) 6592
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    AICD
  • Alt name
    APP intracellular domain
  • Species
    H. sapiens (human)
  • Mutation
    47 aa long
  • Entrez Gene
    APP (a.k.a. AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4)
  • Promoter T7
  • Tag / Fusion Protein
    • MBP-TEV (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminal
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-MBP-TEV-AICD was a gift from Zita Balklava & Thomas Wassmer (Addgene plasmid # 69927 ; http://n2t.net/addgene:69927 ; RRID:Addgene_69927)

  • For your References section:

    APP controls the formation of PI(3,5)P vesicles through its binding of the PIKfyve complex. Currinn H, Guscott B, Balklava Z, Rothnie A, Wassmer T. Cell Mol Life Sci. 2015 Jul 28. 10.1007/s00018-015-1993-0 PubMed 26216398
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