circRAJ31
(Plasmid #69926)

• Purpose: Expresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator.
• Depositing Lab: Alfonso Jaramillo
• Publication: Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. doi: 10.34133/2020/1916789. eCollection 2020.

Backbone

• Vector backbone: pIDTsmartAmp
• Backbone manufacturer: IDT DNA
• Backbone size w/o insert (bp): 2100
• Total vector size (bp): 2584
• Modifications to backbone: BsaI site removed
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: plTet-circRAJ31
• Alt name: Circular RAJ31
• Insert Size (bp): 460
• Promoter: PltetO1-2

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsaI (unknown if destroyed)
• 3′ cloning site: BsaI (unknown if destroyed)
• 5′ sequencing primer: TGTAAAACGACGGCCAGT
• 3′ sequencing primer: GTCATAGCTGTTTCCTG

Resource Information

• Supplemental Documents:
  • 69926_circRAJ31 Genbank file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Engineering a circular riboregulator in Escherichia coli - Rostain et al.
Please check Genbank file for complete sequence annotation.