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pCMV-SPORT6-RNF114-RINGmut
(Plasmid #69922)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69922 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-SPORT6
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4400
  • Total vector size (bp) 5100
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RNF114
  • Alt name
    ZNF313
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    700
  • Mutation
    C29A, C32A
  • Entrez Gene
    RNF114 (a.k.a. PSORS12, ZNF313)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-SPORT6-RNF114-RINGmut was a gift from Francesca Capon (Addgene plasmid # 69922 ; http://n2t.net/addgene:69922 ; RRID:Addgene_69922)

  • For your References section:

    Functional analysis of the RNF114 psoriasis susceptibility gene implicates innate immune responses to double-stranded RNA in disease pathogenesis. Bijlmakers MJ, Kanneganti SK, Barker JN, Trembath RC, Capon F. Hum Mol Genet. 2011 Aug 15;20(16):3129-37. doi: 10.1093/hmg/ddr215. Epub 2011 May 13. 10.1093/hmg/ddr215 PubMed 21571784
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