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pcDNA3-CARD14-E138A
(Plasmid #69921)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69921 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 7600
  • Modifications to backbone
    FLAG tag
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CARD14 isoform 3
  • Alt name
    CARMA2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2200
  • Mutation
    E138A
  • Entrez Gene
    CARD14 (a.k.a. BIMP2, CARMA2, PRP, PSORS2, PSS1)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Pasquale Vito, Universita del Sannio, Italy

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-CARD14-E138A was a gift from Francesca Capon (Addgene plasmid # 69921 ; http://n2t.net/addgene:69921 ; RRID:Addgene_69921)

  • For your References section:

    Activating CARD14 Mutations Are Associated With Generalized Pustular Psoriasis but Rarely Account for Familial Recurrence in Psoriasis Vulgaris. Berki DM, Liu L, Choon SE, David Burden A, Griffiths CE, Navarini AA, Tan ES, Irvine AD, Ranki A, Ogo T, Petrof G, Mahil SK, Duckworth M, Allen MH, Vito P, Trembath RC, McGrath J, Smith CH, Capon F, Barker JN. J Invest Dermatol. 2015 Jul 23. doi: 10.1038/jid.2015.288. 10.1038/jid.2015.288 PubMed 26203641
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