pcDNA3-CARD14-G117S
(Plasmid
#69920)
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PurposeExpresses mutant FLAG-CARD14 in mammalian cells
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 69920 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 7600
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Modifications to backboneFLAG tag
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Vector typeMammalian Expression, Bacterial Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCARD14 isoform 3
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Alt nameCARMA2
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SpeciesH. sapiens (human)
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Insert Size (bp)2200
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MutationG117S
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Entrez GeneCARD14 (a.k.a. BIMP2, CARMA2, PRP, PSORS2, PSS1)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRV (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byPasquale Vito, Universita del Sannio, Italy
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-CARD14-G117S was a gift from Francesca Capon (Addgene plasmid # 69920 ; http://n2t.net/addgene:69920 ; RRID:Addgene_69920) -
For your References section:
Activating CARD14 Mutations Are Associated With Generalized Pustular Psoriasis but Rarely Account for Familial Recurrence in Psoriasis Vulgaris. Berki DM, Liu L, Choon SE, David Burden A, Griffiths CE, Navarini AA, Tan ES, Irvine AD, Ranki A, Ogo T, Petrof G, Mahil SK, Duckworth M, Allen MH, Vito P, Trembath RC, McGrath J, Smith CH, Capon F, Barker JN. J Invest Dermatol. 2015 Jul 23. doi: 10.1038/jid.2015.288. 10.1038/jid.2015.288 PubMed 26203641