pMT-Bip-GFP:V5:KDEL
(Plasmid #69917)

• Purpose: An endoplasmic reticulum (ER) marker used to visulaize the ER structure in Drosophilia S2 cells.
• Depositing Lab: Kenneth Irvine
• Publication: Okajima et al Science. 2005 Mar 11;307(5715):1599-603. Epub 2005 Feb 3.

Backbone

• Vector backbone: pMT/BiP/V5-His/GFP
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4300
• Total vector size (bp): 5260
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP
• Insert Size (bp): 960
• Promoter: pMT
• Tags / Fusion Proteins:
  • Drosophila BiP signal sequence (N terminal on insert)
  • V5 (C terminal on insert)
  • KDEL ER targeting peptide sequence (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: MT-Forward (5'-CATCTCAGTGCAACTAAA-3')
• 3′ sequencing primer: EBV-rev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry