pRK011
(Plasmid
#69875)
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Purposeexpression of Channelrhodopsin-2 T159C mutant
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 69875 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTL412
- Total vector size (bp) 9118
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Vector typeUnspecified
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameChannelrhodopsin-2
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Alt nameChR2
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MutationT159C
- Promoter Drosophila Hsp70
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site AgeI (not destroyed)
- 5′ sequencing primer pCasper-hs
- 3′ sequencing primer SV40-splice-R2 or SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRK011 was a gift from Robert Kittel & Georg Nagel (Addgene plasmid # 69875 ; http://n2t.net/addgene:69875 ; RRID:Addgene_69875) -
For your References section:
Channelrhodopsin-2-XXL, a powerful optogenetic tool for low-light applications. Dawydow A, Gueta R, Ljaschenko D, Ullrich S, Hermann M, Ehmann N, Gao S, Fiala A, Langenhan T, Nagel G, Kittel RJ. Proc Natl Acad Sci U S A. 2014 Sep 23;111(38):13972-7. doi: 10.1073/pnas.1408269111. Epub 2014 Sep 8. 10.1073/pnas.1408269111 PubMed 25201989