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pRK010
(Plasmid #69874)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69874 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTL412
  • Total vector size (bp) 9118
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Channelrhodopsin-2
  • Alt name
    ChR2
  • Mutation
    D156C (XXL)
  • Promoter Drosophila Hsp70

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer pCasper-hs
  • 3′ sequencing primer SV40-splice-R2 or SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Georg Nagel made this construct.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK010 was a gift from Robert Kittel & Georg Nagel (Addgene plasmid # 69874 ; http://n2t.net/addgene:69874 ; RRID:Addgene_69874)

  • For your References section:

    Channelrhodopsin-2-XXL, a powerful optogenetic tool for low-light applications. Dawydow A, Gueta R, Ljaschenko D, Ullrich S, Hermann M, Ehmann N, Gao S, Fiala A, Langenhan T, Nagel G, Kittel RJ. Proc Natl Acad Sci U S A. 2014 Sep 23;111(38):13972-7. doi: 10.1073/pnas.1408269111. Epub 2014 Sep 8. 10.1073/pnas.1408269111 PubMed 25201989
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